Circulating Exosomal miR-144-3p from Crohn’s Disease Patients Inhibits Human Umbilical Vein Endothelial Cell Function by Targeting FN1

Figure 6

miR-144-3p inhibits HUVEC function by targeting FN1 in vitro. A Venn diagram showing the targets predicted by the four bioinformatics software programs (TargetScan 7.2, miRDB, miRmap, and miRwalk) (a). One binding site for miR-144-3p to FN1 was identified by TargetScan 7.2 website prediction (b). The mRNA expression of FN1 was unchanged (c), but FN1 protein expression was lower in miR-144-3p mimic-transfected cells (d). After exogenous addition of the FN1 protein (0.1 μg/μL), the viability of cells was measured by CCK-8 assays (e), and the representative images of wound healing analyses of HUVECs are shown (f). The representative images of the Transwell assay results (g), and, the images of angiogenesis in the indicated cells (h); , , and .

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