[Retracted] PTPN11 Gene Mutations and Its Association with the Risk of Congenital Heart Disease

Figure 4

The effect of PTPN11 variants on ERK1/2 phosphorylation. (a) pERK1/2 was analyzed by transiently expressing the WT and mutant constructs in shRNA knockdown HEK293T cells. Expression of the SHP2-D61N, SHP2-Y63C, SHP2-T73I, SHP2-D192H, SHP2-F285S, and SHP2-N308D mutants resulted in increased ERK1/2 phosphorylation compared with cells expressing WT proteins, and SHP2-Q510E mutation decreased phosphorylation. (b) Quantification of the immunoblot results. . Experiments were repeated three times with three replicates in each experiment.

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