hsa_circ_0003176 Suppresses the Progression of Non-Small-Cell Lung Cancer via Regulating miR-182-5p/RBM5 Axis

Figure 1

hsa_circ_0003176 was downregulated in both NSCLC tissues and cell lines. (a) The expression levels of hsa_circ_0003176 in NSCLC tissues and matched adjacent normal tissues based on GSE101684 datasets (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE101684) and GSE112214 datasets (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi). The GSE101684 data sets contain four NSCLC lung samples and four matched adjacent normal samples (), whose sequencing platform is Arraystar Human CircRNA microarray V2. The GSE112214 data sets contain three NSCLC lung samples and three matched adjacent normal samples (), whose sequencing platform is Agilent-069978 Arraystar Human CircRNA microarray V1. (b) The expression of hsa_circ_0003176 was detected by RT-qPCR in 10 NSCLC tumor tissues and matched adjacent normal. (c) The expression of hsa_circ_0003176 was detected by RT-qPCR in lung cancer cell lines (including H1299, H446, 95-D, and A549) and normal control cell line (16HBE). . (d) ROC curve assay for hsa_circ_0003176 in 10 NSCLC. (e) Characteristics of hsa_circ_0003176 based on circBase and circRNADb databases and detected by RT-qPCR with divergent and convergent primers in cDNA and gDNA, respectively. (f) FISH and nuclear and cytoplasmic RNA fractionation assay were used to detect the localization of hsa_circ_0003176 in A549 and H1299 cells. U6 acts as nuclei control, GAPDH acts as cytoplasmic control, and cicrRNA indicated hsa_circ_0003176. Data was shown as . and .

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