hsa_circ_0003176 Suppresses the Progression of Non-Small-Cell Lung Cancer via Regulating miR-182-5p/RBM5 Axis

Figure 2

hsa_circ_0003176 suppressed the proliferation, migration, and invasion of NSCLC cells. (a) RT-qPCR was used to detect the expression of hsa_circ_0003176 in NSCLC cells (A549 and H1299) transfected with or without overexpressing hsa_circ_0003176 plasmid. (b) CCK-8 assay was used to detect the cell viability in NSCLC cells (A549 and H1299) transfected with or without overexpressing hsa_circ_0003176 plasmid. (c) Colony formation assay was used to detect the colony formation in NSCLC cells (A549 and H1299) transfected with or without overexpressing hsa_circ_0003176 plasmid. (d) Transwell assay was used to detect the cell invasion ability in NSCLC cells (A549 and H1299) transfected with or without overexpressing hsa_circ_0003176 plasmid. (e) The migration in NSCLC cells (A549 and H1299) transfected with or without overexpressing hsa_circ_0003176 plasmid was detected by wound-healing assay. Mock indicated negative control corresponding to hsa_circ_0003176 overexpression plasmid, and circ_0003176 indicated hsa_circ_0003176 overexpression plasmid. Data was shown as . . and .

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