hsa_circ_0003176 Suppresses the Progression of Non-Small-Cell Lung Cancer via Regulating miR-182-5p/RBM5 Axis

Figure 7

hsa_circ_0003176 suppressed the proliferation, migration, and invasion of NSCLC cells by upregulating RBM5 expression. (a) The mRNA expression of RBM5 in NSCLC cell lines (A549 and H1299) transfected with hsa_circ_0003176 overexpression plasmid alone or cotransfected with shRBM5 plasmid was detected using RT-qPCR. (b) The protein expression of RBM5 NSCLC cell lines (A549 and H1299) transfected with hsa_circ_0003176 overexpression plasmid alone or cotransfected with shRBM5 plasmid was detected using western blotting. (c) The viability of NSCLC cell lines (A549 and H1299) transfected with hsa_circ_0003176 overexpression plasmid alone or cotransfected with shRBM5 plasmid was detected using CCK-8 assay. (d) The colony formation ability of NSCLC cell lines (A549 and H1299) transfected with hsa_circ_0003176 overexpression plasmid alone or cotransfected with shRBM5 plasmid was detected using colony formation assay. (e) The migration ability of NSCLC cell lines (A549 and H1299) transfected with hsa_circ_0003176 overexpression plasmid alone or cotransfected with shRBM5 plasmid was detected using wound-healing assay. (f) The invasion ability of NSCLC cell lines (A549 and H1299) transfected with hsa_circ_0003176 overexpression plasmid alone or cotransfected with shRBM5 plasmid was detected using transwell invasion assay. Mock indicated negative control corresponds to hsa_circ_0003176 overexpression plasmid, and shNC indicated negative control corresponds to shRBM5 plasmid. Data was shown as . , , and .

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