circRNA circ_0055724 Inhibits Trophoblastic Cell Line HTR-8/SVneo’s Invasive and Migratory Abilities via the miR-136/N-Cadherin Axis

Figure 4

N-cadherin is a direct target of miR-136. (a) The binding sites of N-cadherin (gene CDH2) and miR-136 was predicted by Starbase, an online bioinformatics tool. (b) Represents the results of luciferase reporter gene experiment carried out in HTR-8/SVneo cells. (c) Compared with miR-NC, overexpression of miR-136 could inhibit luciferase activity in cells, and the inhibition effect disappeared after mutation of the predicted N-cadherin binding site. mRNA and protein expression levels of N-cadherin in miR-136 knockdown or overexpressed HTR-8/SVneo cells were detected by qRT-PCR and WB methods. mRNA and protein expression levels of N-cadherin were increased or decreased by miR-136 knockdown or overexpression, respectively. qRT-PCR and WB methods were used to detect the expression levels of N-cadherin mRNA and protein in HTR-8/SVneo cells in different groups (vector, circ_0055724, circ_0055724+ miR-136). (d) Overexpression of hsa_circ_0055724 increased the expression levels of N-cadherin mRNA and protein, and the expression levels of N-cadherin partially decreased after cotransfection with miR-136. (e) Represents the mRNA expression of N-cadherin in 40 normal tissues and 40 PE tissues detected by qRT-PCR. (f) Correlation analysis of hsa_circ_0055724, miR-136, and N-cadherin expression in PE. .

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