Superficial Dsg2 Expression Limits Epidermal Blister Formation Mediated by Pemphigus Foliaceus Antibodies and Exfoliative Toxins
Figure 1
Suprabasal expression of Dsg2-Flag in newborn Inv-Dsg2 transgenic mice. (a) Histology showing slight epidermal hyperplasia in a newborn Inv-Dsg2 Tg overexpressing Dsg2 in the superficial epidermis under the involucrin promoter, but not in WT mice. (b) Immunostaining of newborn Tg skin with Flag (green) and Dsg2-specific MP6 (red) antibodies showing expression of Dsg2-Flag in the differentiated cell layers of the transgenic epidermis. (c) Immunoblot of WT and Tg skin with MP6 and Flag antibodies, showing Dsg2-Flag in the Tg but not WT skin. Actin was used for equal loading.
