Differential Downregulation of E-Cadherin and Desmoglein by Epidermal Growth Factor

Figure 7

Desmoglein-2 colocalizes with Rab11, a recycling marker. (a) SCC 12F cells were treated for the indicated times with 20 nM EGF, then fixed with 3.7% formaldehyde and permeabilized with 0.1% Triton X-100. Cells were stained with antibodies against both desmoglein-2 and rab11. Secondary antibodies tagged with either FITC or Rhodamine were used. Colocalization is detected by the appearance of yellow staining where the red and green overlap, particularly in EGF-treated cells (white arrows). (b) Mandler’s overlap coefficient, k1, measures the ratio of cadherin colocalized with Rab11 to total cadherin present.