Review Article

Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin

Figure 8

Dermis. (a) 100 μm depth, 760 nm excitation wavelength. Thick and straight collagen fibers show low FLIM values (orange), whereas elastic fibers have high FLIM values and are represented in blue. (b) 100 μm depths, 760 nm excitation wavelength (same spot). Employing the filter excluding SHG signals, curled blue elastic fibers are highlighted and their fine network is visible.
810749.fig.008a
(a)
810749.fig.008b
(b)