Figure 4: Laser-scanning confocal microscopy for internalization of green fluorescent FITC-TSw, respectively, FITC-TSc (50 µg/mL) in HaCaT after incubation of 3 h, 6 h, 12 h, and 24 h. Nuclei were labeled with blue fluorescent DAPI (1 µg/mL). Green spots: FITC-TSw, respectively, FITC-TSc; red spots: Dextran-TexasRed labeled endosomes; yellow spots: Colocalization of FITC-TSw, respectively, FITC-TSc and Dextran-TexasRed labeled endosomes structures. Arrows point to zones of not internalized FITC-labeled xyloglucans.