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Dermatology Research and Practice
Volume 2014, Article ID 736957, 8 pages
http://dx.doi.org/10.1155/2014/736957
Research Article

The Preliminary Study of Effects of Tolfenamic Acid on Cell Proliferation, Cell Apoptosis, and Intracellular Collagen Deposition in Keloid Fibroblasts In Vitro

1Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA
2Department of Plastic and Reconstructive Surgery, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA

Received 30 May 2014; Revised 28 August 2014; Accepted 28 August 2014; Published 22 September 2014

Academic Editor: Lajos Kemény

Copyright © 2014 Dan Yi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. C. Chipev, R. Simman, G. Hatch, A. E. Katz, D. M. Siegel, and M. Simon, “Myofibroblast phenotype and apoptosis in keloid and palmar fibroblasts in vitro,” Cell Death and Differentiation, vol. 7, no. 2, pp. 166–176, 2000. View at Publisher · View at Google Scholar · View at Scopus
  2. R. Simman, H. Alani, and F. Williams, “Effect of mitomycin C on keloid fibroblasts: an in vitro study,” Annals of Plastic Surgery, vol. 50, no. 1, pp. 71–76, 2003. View at Publisher · View at Google Scholar · View at Scopus
  3. C. H. Ricketts, L. Martin, D. T. Faria, G. M. Saed, and D. P. Fivenson, “Cytokine mRNA changes during the treatment of hypertrophic scars with silicone and nonsilicone gel dressings,” Dermatologic Surgery, vol. 22, no. 11, pp. 955–959, 1996. View at Google Scholar · View at Scopus
  4. J. Meenakshi, V. Jayaraman, K. M. Ramakrishnan, and M. Babu, “Ultrastructural differentiation of abnormal scars,” Annals of Burns and Fire Disasters, vol. 18, pp. 83–88, 2005. View at Google Scholar
  5. H. Wang and S. Luo, “Establishment of an animal model for human keloid scars using tissue engineering method,” Journal of Burn Care and Research, vol. 34, no. 4, pp. 439–446, 2013. View at Publisher · View at Google Scholar · View at Scopus
  6. A. Asilian, A. Darougheh, and F. Shariati, “New combination of triamcinolone, 5-fluorouracil, and pulsed-dye laser for treatment of keloid and hypertrophic scars,” Dermatologic Surgery, vol. 32, no. 7, pp. 907–915, 2006. View at Publisher · View at Google Scholar · View at Scopus
  7. C.-C. E. Lan, I.-H. Liu, A.-H. Fang, C.-H. Wen, and C.-S. Wu, “Hyperglycaemic conditions decrease cultured keratinocyte mobility: implications for impaired wound healing in patients with diabetes,” The British Journal of Dermatology, vol. 159, no. 5, pp. 1103–1115, 2008. View at Publisher · View at Google Scholar · View at Scopus
  8. T. T. Phan, I. J. Lim, O. Aalami et al., “Smad3 signalling plays an important role in keloid pathogenesis via epithelial-mesenchymal interactions,” Journal of Pathology, vol. 207, no. 2, pp. 232–242, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. Y. Yagi, E. Muroga, M. Naitoh et al., “An ex vivo model employing keloid-derived cell-seeded collagen sponges for therapy development,” Journal of Investigative Dermatology, vol. 133, no. 2, pp. 386–393, 2013. View at Publisher · View at Google Scholar · View at Scopus
  10. F. Syed, D. Sherris, R. Paus, S. Varmeh, P. P. Pandolfi, and A. Bayat, “Keloid disease can be inhibited by antagonizing excessive mTOR signaling with a novel dual TORC1/2 inhibitor,” The American Journal of Pathology, vol. 181, no. 5, pp. 1642–1658, 2012. View at Publisher · View at Google Scholar · View at Scopus
  11. F. Syed and A. Bayat, “Notch signaling pathway in keloid disease: enhanced fibroblast activity in a Jagged-1 peptide-dependent manner in lesional vs. extralesional fibroblasts,” Wound Repair and Regeneration, vol. 20, no. 5, pp. 688–706, 2012. View at Publisher · View at Google Scholar · View at Scopus
  12. W. J. Lee, I.-K. Choi, J. H. Lee et al., “Relaxin-expressing adenovirus decreases collagen synthesis and up-regulates matrix metalloproteinase expression in keloid fibroblasts: in vitro experiments,” Plastic and Reconstructive Surgery, vol. 130, no. 3, pp. 407e–417e, 2012. View at Publisher · View at Google Scholar · View at Scopus
  13. J. Krajickova, V. Pesakova, M. Adam, and K. E. Senius, “Effect of tolfenamic acid on the metabolism of the main connective tissue components in rats,” Arzneimittel-Forschung, vol. 37, no. 2, pp. 177–180, 1987. View at Google Scholar · View at Scopus
  14. J.-H. Kim, J.-Y. Jung, J.-H. Shim et al., “Apoptotic effect of tolfenamic acid in KB human oral cancer cells: possible involvement of the p38 MAPK pathway,” Journal of Clinical Biochemistry and Nutrition, vol. 47, no. 1, pp. 74–80, 2010. View at Publisher · View at Google Scholar · View at Scopus
  15. P. D. Gotzsche, “Meta-analysis of grip strength: most common, but superfluous variable in comparative NSAID trials,” Danish Medical Bulletin, vol. 36, no. 5, pp. 493–495, 1989. View at Google Scholar · View at Scopus
  16. P. C. Gotzsche, “Review of dose-response studies of NSAIDs in rheumatoid arthritis,” Danish Medical Bulletin, vol. 36, no. 4, pp. 395–399, 1989. View at Google Scholar · View at Scopus
  17. P. C. Gotzsche, “Methodology and overt and hidden bias in reports of 196 double-blind trials of nonsteroidal antiinflammatory drugs in rheumatoid arthritis,” Controlled Clinical Trials, vol. 10, no. 1, pp. 31–56, 1989. View at Publisher · View at Google Scholar · View at Scopus
  18. S. Rossi, W. Ou, D. Tang et al., “Gastrointestinal stromal tumours overexpress fatty acid synthase,” Journal of Pathology, vol. 209, no. 3, pp. 369–375, 2006. View at Publisher · View at Google Scholar · View at Scopus
  19. E.-S. Choi, J.-H. Shim, J.-Y. Jung et al., “Apoptotic effect of tolfenamic acid in androgen receptor-independent prostate cancer cell and xenograft tumor through specificity protein 1,” Cancer Science, vol. 102, no. 4, pp. 742–748, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. S. U. Kang, Y. S. Shin, H. S. Hwang, S. J. Baek, S.-H. Lee, and C.-H. Kim, “Tolfenamic acid induces apoptosis and growth inhibition in head and neck cancer: involvement of NAG-1 expression,” PLoS ONE, vol. 7, no. 4, Article ID e34988, 2012. View at Publisher · View at Google Scholar · View at Scopus
  21. D. Eslin, U. T. Sankpal, C. Lee et al., “Tolfenamic acid inhibits neuroblastoma cell proliferation and induces apoptosis: a novel therapeutic agent for neuroblastoma,” Molecular Carcinogenesis, vol. 52, no. 5, pp. 377–386, 2013. View at Publisher · View at Google Scholar · View at Scopus
  22. M. Abdelrahim, C. H. Baker, J. L. Abbruzzese, and S. Safe, “Tolfenamic acid and pancreatic cancer growth, angiogenesis, and Sp protein degradation,” Journal of the National Cancer Institute, vol. 98, no. 12, pp. 855–868, 2006. View at Publisher · View at Google Scholar · View at Scopus
  23. S.-H. Lee, H. B. Jae, K. C. Chang et al., “ESE-1/EGR-1 pathway plays a role in tolfenamic acid-induced apoptosis in colorectal cancer cells,” Molecular Cancer Therapeutics, vol. 7, no. 12, pp. 3739–3750, 2008. View at Publisher · View at Google Scholar · View at Scopus
  24. M. Abdelrahim, C. H. Baker, J. L. Abbruzzese et al., “Regulation of vascular endothelial growth factor receptor-1 expression by specificity proteins 1, 3, and 4 in pancreatic cancer cells,” Cancer Research, vol. 67, no. 7, pp. 3286–3294, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. U. T. Sankpal, M. Abdelrahim, S. F. Connelly et al., “Small molecule tolfenamic acid inhibits PC-3 cell proliferation and invasion in vitro, and tumor growth in orthotopic mouse model for prostate cancer,” Prostate, vol. 72, no. 15, pp. 1648–1658, 2012. View at Publisher · View at Google Scholar · View at Scopus
  26. D.-L. Fan, W.-J. Zhao, Y.-X. Wang, S.-Y. Han, and S. Guo, “Oxymatrine inhibits collagen synthesis in keloid fibroblasts via inhibition of transforming growth factor-β1/Smad signaling pathway,” International Journal of Dermatology, vol. 51, no. 4, pp. 463–472, 2012. View at Publisher · View at Google Scholar · View at Scopus
  27. C. K. Lim, A. S. Halim, N. S. Yaacob, I. Zainol, and K. Noorsal, “Keloid pathogenesis via Drosophila similar to mothers against decapentaplegic (SMAD) signaling in a primary epithelial-mesenchymal in vitro model treated with biomedical-grade chitosan porous skin regenerating template,” Journal of Bioscience and Bioengineering, vol. 115, no. 4, pp. 453–458, 2013. View at Publisher · View at Google Scholar · View at Scopus
  28. C.-S. Wu, P.-H. Wu, A.-H. Fang, and C.-C. E. Lan, “FK506 inhibits the enhancing effects of transforming growth factor (TGF)-β1 on collagen expression and TGF-β/Smad signalling in keloid fibroblasts: implication for new therapeutic approach,” The British Journal of Dermatology, vol. 167, no. 3, pp. 532–541, 2012. View at Publisher · View at Google Scholar · View at Scopus