Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique

Figure 1

Experimental design of the cell culture part of the study. Fibroblasts were seeded as a confluent layer and cultivated with ascorbate for 14 days. A reseeding of fibroblasts took place: without modification for standard condition, with a flip of the matrix sheet before the seeding for the “flipped” condition, and with the elevation of the matrix sheet on a permeable ring of plastic for the “stretched” condition. All the cultures were pursued for 14 days.