Effects of Topical Emu Oil on Burn Wounds in the Skin of Balb/c Mice
Table 1
Microscopic features of wounds in Emu oil group and control group in different days.
Day 4
Day 7
Day 10
Day 14
Case
Control
Case
Control
Case
Control
Case
Control
Inflammatory cell density (cell/HPF)
145 ± 56 Cell/HPF (++++)
98 ± 35 Cell/HPF (++++/+++)
91 ± 33 Cell/HPF (+++/+++)
72 ± 31 Cell/HPF (+++/++)
67 ± 28 Cell/HPF (+++/++)
29 ± 15 Cell/HPF (++/+)
21 ± 14 Cell/HPF (+)
12 ± 9 Cell/HPF (+/−)
Inflammatory cell type
100% PMN, no MN
98% PMN, 2% MN
93% PMN, 7% MN
70% PMN, 30% MN
80% PMN, 20% MN
5% PMN, 95% MN
30% PMN, 70% MN
No PMN, 100% MN
Granulation tissue formation
—
−/+
+/−
+/−
—
Edema
/+
+/−
+/−
—
Fibroblast density
3 ± 2/HPF
5 ± 2/HPF
9 ± 4/HPF
13 ± 7/HPF
23 ± 12/HPF
18 ± 8/HPF
22 ± 10/HPF
20 ± 11/HPF
Fibroblast activity
—
—
−/+
+/
++
Basal layer
Damaged
Damaged
Marked in margins
Marked in margins
Join completed
Join completed
Completed
Completed
Increased thickness of epidermis in the edge of wound
No epidermis in the edge
No epidermis in the edge
4 ± 1 layers’ epidermis
4 ± 1 layers’ epidermis
2 ± 1 layers’ epidermis
2 ± 1 layers’ epidermis
1 ± 1 layers’ epidermis
No epidermis in the edge
Keratosis in the edge of wound
2 ± 1 layers
2 ± 1.5 layers
3 ± 1.5 layers
2 ± 1 layers
6 ± 2 layers
2 ± 1 layers
3 ± 1 layers
2 ± 1 layers
Number of hair follicles in the scar
12 ± 5 necrotic follicles/LPF
15 ± 6 necrotic follicles/LPF
0
0
0
0
0
0
Number of hair follicles in the margins
20 ± 2
20 ± 2.5
22 ± 3
18 ± 2.6
25 ± 3.4
15 ± 1.4
28 ± 2.6
12 ± 1.4
Microscope: Olympus SZX research microscope. LPF (Low Power Field) is 100x and HPF (High Power Field) is 400x. Diameter of objective lens of LPF is 1.02 mm and HPF is 0.51 mm.
