Table 3: Macroscopic and microscopic characteristics of fungi isolated from patients attending Kampala International University Teaching Hospital, Western Campus, Uganda.

No.Culture Morphology on SDAMicroscopic examinationFungi identified

1.The colonies were suede-like and few powdery with a flat, raised and folded centre with concentric furrow. The colour may vary from white, beige, grayish, and pale yellow pale-buff to yellow to dark-brown. The reverse appeared as yellow-brown to reddish-brown.It had branched hyphae which were relatively wide and had numerous septa. It had numerous microconidia with varying sizes from long clavate to broad pyriform that were borne at right angles to the hyphae typical of “birds on a wire”.
Very occasional few macroconidia were present on some cultures and appeared as smooth, thin-walled, irregular, and clavate shaped.
Swollen giant forms of microconidia and chlamydospores were produced in older cultures.
Trichophyton tonsurans

2.The colonies were flat, greyish-white to light tan-white in colour, and had a dense suede-like to downy surface that spread on entire culture. The reverse appeared yellow-brown to reddish-brown in colour.Its hyphae were septate and exhibited a pectinate (comb-like) and racquet like structures with having thick-walled terminal or intercalary chlamydospores. The terminal chlamydospores were uniquely tapered at their apical end.
Macroconidia and microconidia were not seen.
Microsporum audouinii

3.The colonies had a powdery to granular surface and appeared white to cream in colour which gradually turned yellowish. A few cultures had raised central tufts with foldings. The reverse appeared yellow-brown then later
reddish-brown in colour.
Its hyphae were septate and some were spiral shaped at the end. Few hyphae had chlamydospores that were spherically shaped.
Numerous single celled microconidia that were subspherically shaped and few with clavate to pyriform were formed in clusters. Multicelled macroconidia that were thin-walled and had clavate-shape also were present.
Trichophyton mentagrophytes

4.The colonies were flat and slightly raised, appeared white to cream, and became rose on aging and became suede-like to down; its reverse appeared yellow-brown to wine-red in colour.It had a hyaline septate hyphae with numerous chlamydospores only in older cultures. Macroconidia were only seen in a few older cultures that appeared thin-walled multiseptate and slender cylindrical.
It had numerous clavate to pyriform shaped microconidia.
Trichophyton rubrum

5.The colonies appeared flat, with a dense cottony, fluffy-hairy surface having radiating furrow. Its obverse exhibited a white to cream colour with light yellowish pigment at its periphery. The reverse was pigmented with pale tan to yellowish which turned brownish on aging.It had a septate hyphae with numerous macroconidia. The macroconidia were multicelled (6-14 cells), long, typically spindle-shaped, thick-walled with an echinulate texture. The terminal apex of the macroconidia was tapered into a knob like end. A few pyriform to clavate microconidia was rarely seen and appeared club shaped formed along the length of the hyphae.Microsporum canis

6.Colonies (SDA) are slow growing, small, button or disc-shaped, white to cream coloured, with a suede-like to velvety surface, a raised centre, and flat periphery with some submerged growth. Reverse pigment may vary from non-pigmented to yellow.Chlamydospores are often in chains. The tips of some hyphae are broad and club-shaped, and occasionally divided, giving the so-called “antler” effect.
Trichophyton verrucosum showing clavate to pyriform microconidia, characteristic rat tail or string bean-shaped macroconidia, terminal vesicles at the tips of hyphae in young colonies and chains of chlamydospores.
Trichophyton verrucosum

7.The colonies appeared folded, glabrous, and mounded with deep violet in colour.No conidia are usually seen.
The hyphae were seen moderately broad with numerous branches. The hyphae from older cultures had significant number of chlamydospores present.
Trichophyton violaceum

8.The colonies were fast growing, flat, granular, and few radial groves. The observe varied in colour from white, yellow, yellow-brown, brown to black, or dark yellow-green on aging. However, other colonies were classically unadorned green in colour. The reverse of each colony varied from greyish to purple-brown.Conidial heads are short columnar and biseriate. Stipes are usually short, brownish, and smooth-walled. Conidia are globose and rough-walled.
It had short biseriate conidial heads with flask shaped phialides borne on metulae. These were slightly spherical where conidia were produced.
Conidia were globose (shperical) in shape.
Aspergillus spp

9.The colonies were medium sized and appeared white to cream-coloured with a smooth, waxy, and glabrous surface with a yeast-like smell.The yeast cells were spherical to subspherical in shape (budding blastoconidia) with a pseudohyphae. With India Ink Preparation, no capsules were present.
It produced germ tubes when incubated in 0.5 mL of serum containing 0.5% glucose at 35°C for 2-3 hours.
Candida albicans

10.The colonies were fast growing as pink with abundant cottony/hair like mycelium.It had septate hyphae. Macroconidia were absent. Microconidia were spindle shaped, rounded, and tapered at each end, contained one/three cells. It had numerous intercalary chlamydospores which were ovoidal in shape while a few were ellipsoidal in shape.Fusarium spp.