Gene Expression of CD70 and CD27 Is Increased in Alopecia Areata Lesions and Associated with Disease Severity and Activity

Table 1

Primers used for real-time polymerase reaction assay.

Gene	Sequence
CD70	Forward: 5′- TTGGTGATCTGCCTCGTGGT-3′
	Reverse: 5′- CCTTGTCCAGCTCTGGTCCAT-3′
CD27	Forward: 5′- ACCCTCAGCCCACCCACTTA-3′
	Reverse: 5′- CAGGGTGAAAACAAGGAACATT-3′
GAPDH	Forward: 5′-CGGAGTCAACGGATTTGGTCGTAT- 3′
	Reverse: 5′-AGCCTTCTCCATGGTGGT-3′

CD: cluster differentiation; GAPDH : human glyceraldehyde-3-phosphate dehydrogenase.