Anti-Hyperglycemic Properties of Crude Extract and Triterpenes from Poria cocos
Figure 4
Effects of the P. cocos crude extract, dehydrotumulosic acid (DHTuA), and pachymic acid (PA) on PPAR- activation. (a) 3T3-L1 cells were transiently transfected with reporter constructs, pSGGAL4-PPAR- (2 g), and (UAS)5-tk-LUC plasmid (10 g). The cells were treated with DMSO (N: negative control), rosiglitazone (RSG: 1 M), and the P. cocos crude extract (0.063, 0.25, and 0.63 mg/ml). After 24 h, cell lysates were subjected to dual luciferase assay. (b) The same procedure as (a) except that the cells were treated with dehydrotumulosic acid (DHTuA: 10, 40, or 100 M) and pachymic acid (PA: 10, 40, or 100 M). All the data in folds are expressed as P; () less than .05 is considered to be statistically significant.
