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Evidence-Based Complementary and Alternative Medicine
Volume 2011 (2011), Article ID 140462, 8 pages
http://dx.doi.org/10.1155/2011/140462
Research Article

Dieckol from Ecklonia cava Regulates Invasion of Human Fibrosarcoma Cells and Modulates MMP-2 and MMP-9 Expression via NF-κB Pathway

1Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea
2Key Laboratory of Molecular Enzymology and Enzyme Engineering of Ministry Education, College of Life Science, Jilin University, Changchun 130021, China
3Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea
4School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, China

Received 28 December 2010; Revised 17 March 2011; Accepted 3 June 2011

Copyright © 2011 Chen Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The matrix metalloproteinase (MMP) family is involved in the breakdown of extracellular matrix in normal physiological processes, as well as in the disease processes such as arthritis and cancer metastasis. In the present study, dieckol was obtained with high yield from marine brown alga Ecklonia cava (EC), and its effect was assessed on the expression of MMP-2 and -9 and morphological changes in human fibrosarcoma cell line (HT1080). Dieckol inhibited the expression of MMP-2 and -9 in a dose-dependent manner and also suppressed the cell invasion and the cytomorphology in 3D culture system on HT1080 cells. Moreover, dieckol may influence nuclear factor kappa B (NF-κB) pathway without obvious influence on activator protein-1 (AP-1) pathway and tissue inhibitor of metalloproteinases (TIMPs). In conclusion, dieckol could significantly suppress MMP-2 and -9 expression and alter cytomorphology of HT1080 cell line via NF-κB pathway.