Original Article

Analyzing Cytotoxic and Apoptogenic Properties of Scutellaria litwinowii Root Extract on Cancer Cell Lines

Figure 6

Flow cytometry histograms of apoptosis assays by PI method in HeLa cells. Cells were treated with 50  g m of CH2Cl2 fraction for 24 h. Sub-G1 peak as an indicative of apoptotic cells, was induced in CH2Cl2 fraction treated but not in control cells. (1) control, (2) serum free (positive control); and (3) CH2Cl2 fraction. Flow cytometry histogram of positive control in which cells were cultured in serum free medium and CH2Cl2 fraction-treated cells. CH2Cl2 fraction-treated cells exhibited a sub-G1 peak in HeLa cells that indicates the involvement of an apoptotic process in CH2Cl2 fraction-induced cell death.
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