Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage
Figure 3
Expressions of three germ layer markers in RG-treated, differentiated cells. After 20 days of treatment, expression of early ectoderm, mesoderm, and endoderm markers were evaluated. RG-treated differentiated cells showed more dominant expression of mesodermal marker, Brachyury and endodermal marker, HNF3. (a) Morphological observation of differentiating cells in suspension and attached state. (b) Expressions of three germ layer markers in reattached, RG-treated cells. (c) Immunostaining results of ectoderm (Nestin), mesoderm (Brachyury) and endoderm (HNF3).