Antidiabetic Activities of Abutilon indicum (L.) Sweet Are Mediated by Enhancement of Adipocyte Differentiation and Activation of the GLUT1 Promoter

Figure 6

The effect of A. indicum crude extract on the transcription level of Glut1. L6 myocytes were transfected with GLUT1 promoter and pRL-SV40 plasmid. Then, cells were exposed to indicate concentrations of crude extract for 16 h as described in the ‘Methods’ section. The luciferase activity was expressed as a ratio of firefly to renilla luciferase activity. Transfections and assays were performed in triplicate for each experiment group. Data showed mean ± SEM of fold increase of three replicates against control group. *P < .05 compared with untreated sample group. The pGL3-Basic vector (empty) was used as a negative control, and pGL3-control vector was used for a positive control.