Cytotoxic Activities of Physalis minima L. Chloroform Extract on Human Lung Adenocarcinoma NCI-H23 Cell Lines by Induction of Apoptosis
Figure 6
(a) Time course mRNA expression of β-actin, c-myc, caspase-3 and p53 in NCI-H23 cells incubated in the absence or presence of P. minima chloroform extract. β-actin was used as an internal standard control for each PCR reation. (b) Semi-quantitative analysis of the c-myc, caspase-3 and p53 mRNA level in NCI-H23 treated with P. minima chloroform extract using densitometric scanning. Each value represented mean ± SEM ( at each point) of the ratio of RT-PCR product of the respective genes to β-actin, assigning the ratio in unstimulated cells as 1. versus C (control).
