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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 193842, 9 pages
http://dx.doi.org/10.1155/2011/193842
Research Article

Rottlerin Inhibits Lonicera japonica-Induced Photokilling in Human Lung Cancer Cells through Cytoskeleton-Related Signaling Cascade

1School of Chinese Medicine Resources, China Medical University, 91, Hsueh-Shih Road, Taichung 40402, Taiwan
2Graduate Institute of Integrated Medicine, China Medical University, 91, Hsueh-Shih Road, Taichung 40402, Taiwan
3School of Pharmacy, China Medical University, 91, Hsueh-Shih Road, Taichung 40402, Taiwan
4Department of Internal Medicine, Chang-Hua Hospital, 80, Chung-Cheng Road Sec. 2, Puhsin 51341, Chang-Hua, Taiwan

Received 23 September 2010; Revised 11 December 2010; Accepted 5 January 2011

Copyright © 2011 Bang-Jau You et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study demonstrated that many apoptotic signaling pathways, such as Rho family, PKC family, MAP kinase family, and mitochondria-mediated apoptotic pathway, were triggered by Lonicera japonica extracts and irradiation in CH27 cells. Rottlerin, a PKCδ -selective inhibitor, reversed the photoactivated Lonicera japonica extract-induced decrease in PKCδ protein expression and change in cell morphology in this study. In addition, rottlerin inhibited the photoactivated Lonicera japonica-induced decrease in protein expression of Ras, ERK, p38, PKCα, and PKCε, which are the kinases of prosurvival signaling pathway. We also demonstrated that pretreatment with rottlerin prevented actin microfilaments and microtubules from damage during the photoactivated Lonicera japonica-induced CH27 cell death. Furthermore, the promotion of the cytoskeleton-related signaling cascade following rottlerin by upregulation of cytoskeleton-related mediators (p38, HSP27, FAK, paxillin, and tubulin) and molecules of downstream of F-actin (mitochondria-mediated apoptosis pathway) reduces CH27 cell death, indicating that cytoskeleton is the potential target in the photoactivated Lonicera japonicaextract-induced photokilling of CH27 cells.