Rottlerin Inhibits Lonicera japonica-Induced Photokilling in Human Lung Cancer Cells through Cytoskeleton-Related Signaling Cascade
Figure 4
(a) Effects of photoactivated Lonicera japonica extracts on the expression of cytoskeleton-related proteins in CH27 cells. Cells were incubated with 0.1% ethanol or 100 μg/mL Lonicera japonica extracts for 0, 2, 4, and 6 h and then irradiated with 0.8 J/cm2 fluence dose. (b) The effect of rottlerin on the photoactivated Lonicera japonica extract-induced changes in cytoskeleton-related proteins expression of CH27 cells. Cells were pretreated with 10 μM rottlerin for 1 h and then treated with 0.1% ethanol or 100 μg/mL Lonicera japonica extracts for 4 h and 0.8 J/cm2 fluence dose. After irradiation, cell lysates were analyzed by 5% (FAK), 9% (paxillin), 10% (tubulin), and 13% (HSP27) SDS-PAGE and then probed with primary antibodies as described in Section 2. −: control cells; +: Lonicera japonica extract- or rottlerin-treated cells. Results are representative of three independent experiments.
