Safety of Herbal Medicinal Products: Echinacea and Selected Alkylamides Do Not Induce CYP3A4 mRNA Expression

Table 1

β-actin, GAPDH and CYP3A4 primers.

Gene	Gene accession no.	Primer	Sequence	Concentration (nM)	Product size
β-Actin	X00351	Forward	5^′-TCAGCAAGCAGGAGTATG-3^′	300	97
		Reverse	5^′-GTCAAGAAAGGGTGTAACG-3^′	300
GAPDH	NM_002046	Forward	5^′-TCTCTGCTCCTCCTGTTC-3^′	900	120
		Reverse	5^′-GCCCAATACGACCAAATCC-3^′	900
CYP3A4	NM_017460	Forward	5^′-ATCATTGCTGTCTCCAACCTTCAC-3^′	200	103
		Reverse	5^′-TGCTTCCCGCCTCAGATTTCTC-3^′	200

Genebank accession numbers, primer sequences, concentrations and product sizes are shown all genes.