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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 213628, 8 pages
http://dx.doi.org/10.1093/ecam/neq071
Original Article

Antiatherogenic Potential of Nigella sativa Seeds and Oil in Diet-Induced Hypercholesterolemia in Rabbits

1Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia
2Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia
3Department of Food Science & Technology, Faculty of Agriculture, University of Sana’a, Sana’a, Yemen
4Laboratory of Cancer Research MAKNA-UPM, Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia
5Department of Biochemistry and Molecular Biology, Faculty of Medicine and Health Sciences, University of Sana’a, Sana’a, Yemen

Received 2 April 2010; Accepted 20 May 2010

Copyright Ā© 2011 Ghanya Al-Naqeep et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nigella sativa or Black seed (N. sativa L.) is traditionally used for several ailments in many Middle Eastern countries. It is an annual herbaceous plant that belongs to the Ranuculacea family with many beneficial properties as antitumor, antidiabetic, antihypertensive, antioxidative and antibacterial. This work attempted to study the effect of N. sativa seeds powder and oil on atherosclerosis in diet-induced hypercholesterolemic (HC) rabbits in comparison with simvastatin (ST). Twenty-five adult New Zealand male white rabbits, weighing 1.5–2.5 kg, were divided into five groups; normal group (NC, š‘› = 5 ) and four hypercholesterolemic groups ( š‘› = 2 0 ): a positive control (PC) and three HC groups force fed diet supplemented with 1000 mg  K g āˆ’ 1 body weight of N. sativa powder (NSP), 500 mg  K g āˆ’ 1 body N. sativa oil (NSO) and 10 mg  K g āˆ’ 1 ST for 8 weeks. Feeding HC rabbits with N. sativa either in powder or oil forms was shown to significantly reduce ( š‘ƒ < . 0 5 ) total cholesterol (TC) and low-density lipoprotein cholesterol (LDLC) levels and enhance high-density lipoprotein cholesterol (HDL) levels after treatment for 2, 4, 6 and 8 weeks compared to the PC group. Plaque formation was significantly inhibited while the intima: media ratio was significantly reduced in the NSP and NSO supplemented groups compared to the PC group. In conclusion, treatment of HC rabbits with N. sativa seeds powder or oil showed hypocholesterolemic and antiatherogenic cardioprotective properties.