Research Article

Resequencing DCDC5 in the Flanking Region of an LD-SNP Derived from a Kidney-Yang Deficiency Syndrome Family

Table 1

Primers designed for the resequencing of DCDC5. A website primer design platform, Primer3, was employed under the default primer selection conditions to design these primers based on the genbank accession number NM_198462.2. All samples, including twelve KDS patients and three spouses from the KDS family were resequenced and electrophoresed on an ABI 377 automated sequencers.

ExonForward primerReward primer

1taaccccttcaggtgagcagcaatcagtcacctggccttt
2ggagaagcaggagcctttctgtatcaaaaccagggccaag
3aacattgccgtcatttcacaactgccttgcaaaaacacct
4ccagcaatagtgctatacaataggaaatggccccatatggttttct
5ccccaatgactgctggtactccagctacctgaaaggctga
6caggtggggagagagagagaaataccaacggcagagatgg
7ggctcgctgactgctaagtttgttgaaggccaacatttca
8gccaggctatctggaaaatggggaattgctgggttgtcta
9tcctctctttccagttggatgacgtggccaaaagaaaagaa
10ttggccacgtacaaaggactctacgcttgaaagccaaagg
11tttgccttaatgctttcctgagaccacacaactgggcctta
12ttccaagttcattcggttctaatatgggagcccttttgct
13gccacaatttggagaggaaatccagcctgggtatcagagt
14tgttgcctttatcagcagtttgagcaagatgcagaggtgaaa
15ggactgggacttccactgatttcacaaacttggcatgagc
16ccaccaacatcctgtggaatcaggaggcaaaggtggataa
17tggagctctcaccaacagaaccaggagatcagagggaaca
18cccttcatcctgcagtcttccttgccattgggtttgattt
19tgtcactgtgtggtgggattatttcactcaaaaatcagcattca
20tcattggtgtcgaattgtcgcaatggggaaaaggaaatca
21gccagaaacacaagccagataatctggccagggacattta
22cagctggattggactactgttgttgacactcttcgggtcaca
23gattttggcattgcctgtttggcaaatgtgcaaacatgag
24tgccttaagccctgtcatctttgcccaggtatcctcctaa
25gctcctgtgtggaacgatttgctgggcctttttctcttct
26aagcaaggggaatttaccagatctgctggacaagttgcctat
27acatttgcatgcccttcttcgcgattgaattctccagagc
28ggatttgtgaggggtcttcacaaggctgcagtaagctgtg
29gtgtgtgcgtgtgttcatgttcaaaataggcccattgaaaa