Protective Mechanism of KIOM-4 in Streptozotocin-Induced Pancreatic β-Cells Damage Is Involved in the Inhibition of Endoplasmic Reticulum Stress
Figure 1
The effects of KIOM-4 on STZ-induced cell damage. RINm5F cells were treated with KIOM-4 at 50 g/mL, after 1 hour, 10 mM of STZ was added and was incubated for 24 hours. (a) Cell viability was measured using MTT assay. *Significantly different from control cells (). **Significantly different from STZ-treated cells (). (b) Apoptotic body formation was observed under a fluorescence microscope after Hoechst 33342 staining (original magnification, x400). Arrows indicate apoptotic bodies. (c) DNA fragmentation was quantified with an ELISA kit. Measurements were made in triplicate and the values are expressed as means SEM. *Significantly different from control cells () and **significantly different from STZ-treated cells ().
