Gingyo-San Enhances Immunity and Potentiates Infectious Bursal Disease Vaccination

Table 1

Oligonucleotide primer sets used for semi-quantitative RT-PCR.

RNA target	Primer sets	Oligonucleotide sequence	Annealing temperature (°C)	Product size (bp)	Accession no.
INF-γ	F	5′-AGCTGACGGTGGACCTATTA-3′	56	259	NM_205149
	R	5′-GGCTTTGCGCTGGATTCTC-3′
IL-2	F	5′-CTGGGACCACTGTATGCTCT-3′	55	256	AF017645
	R	5′-CACCAGTGGGAAACAGTATC-3′
IL-4	F	5′-ACCCAGGGCATCCAGAAG-3′	55	258	AJ621735
	R	5′-CAGTGCCGGCAAGAAGTT-3′
IL-12	F	5′-AGACTCCAATGGGCAAATGA-3′	56	244	NM_213571
	R	5′-CTCTTCGGCAAATGGACAGT-3′
GAPDH	F	5′-GGTGGTGCTAAGCGTGTTAT-3′	56	264
	R	5′-ACCTCTGTCATCTCTCCACA-3′