Immunohistochemical Evaluation of p63, E-Cadherin, Collagen I and III Expression in Lower Limb Wound Healing under Honey

Figure 3

Immunohistochemical photomicrographs (40x oil, a-p) of skin biopsies (normal and leg wound periphery): (a)–(d) depicted expression of p63 in intact (a), before (b), after 15th days (c), and 22nd days (d) of topical intervention of honey: (e)–(h) demonstrated E-cadherin expression in different cellular layers of epithelium in normal (e) skin and in peripheral biopsies of before (f) and after said days of interventions (g), (h); (i)–(p) depicted expression of Collagen I (i)–(l) and Collagen III (m)–(p) in normal skin (i), (m), preintervention (j), (n) and in early (k), (o) and late (l), (p) periods of postintervention. The expression of p63 (b), E-cadherin (f), collagen I (j) and III (n) in flattened epithelium in preintervention stage and their changed expression status in normal and postintervention periods-p63⁺ nuclei distribution (a), (c), and (d), increased E-cadherin expression (e), (g), and (h), altered Collagen I (i), (k), and (l) and Collagen III (m), (o), and (p) density, orientation, and distribution.

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