Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells
Figure 4
Bufalin induced ROS-dependent Bax translocation. (a) Typical fluorescence images of cells expressing GFP-Bax after different treatments. Mitotracker was used to label mitochondria. Scale bar: 10 μm. (b) Percentage of cells with Bax translocation at 0, 6 or 12 h after STS treatment. (c) Percentage of cells with Bax translocation at 0, 12, 24 or 48 h after bufalin treatment in the absence or presence of NAC. Data were collected in 400–500 cells per treatment in 15–20 randomly selected image frames and from three independent experiments (n = 3). Results were presented as mean ± SD. *P > .05, compared with control. **P <.05, compared with control. #P <.05, compared with treatment with bufalin alone.
