Inhibitory Effects of Ethyl Acetate Extract of Andrographis paniculata on NF-κB Trans-Activation Activity and LPS-Induced Acute Inflammation in Mice

Figure 2

Effects of EtOAc extract of AP on survival rate of mice treated with LPS endotoxin. The mice were divided into the following groups: the PBS, LPS and PDTC groups and the AP 1, 2, 3, 4 groups (tube-feeding 0.78, 1.56, 3.12 and 6.25 mg kg⁻¹ BW AP EtOAc extract in 100 μl soybean oil/day, resp.). After 1-week tube-feeding of AP EtOAc extract, all mice were i.p. injected with 15 mg kg⁻¹ BW LPS to induce acute inflammation. Mice in the PDTC group were i.p. injected with 50 mg kg⁻¹ BW PDTC 1 h before LPS administration. Statistical comparison between different survival curves was analyzed by Cox's proportional hazards regression test. Statistical analysis was performed using the Student's t-test for multiple comparisons (Asterisk indicates the significant as compared with LPS alone, P < .05, P < .01).