Inhibitory Effects of Ethyl Acetate Extract of Andrographis paniculata on NF-κB Trans-Activation Activity and LPS-Induced Acute Inflammation in Mice

Figure 3

Effects of EtOAc extract on endotoxin induced MIP-2 mRNA expression in brain of BALB/c mice. The mice were divided into the following groups: the PBS, LPS and PDTC groups and the AP 1, 2, 3 groups (tube-feeding 0.78, 1.56 and 3.12 mg kg⁻¹ BW AP EtOAc extract in 100 μl soybean oil/day, resp.). After 1-week tube-feeding of AP EtOAc extract, all mice were i.p. injected with 15 mg kg⁻¹ BW LPS to induce acute inflammation. Mice in the PDTC group were i.p. injected with 50 mg kg⁻¹ PDTC 1 h before LPS administration. Results of MIP-2/β-actin mRNA ratio are expressed as mean ± SEM (n = 8). Statistical analysis was performed using Student's t-test for multiple comparisons (Asterisk indicates the significant as compared with LPS alone, P < .05).