Inhibitory Effects of Ethyl Acetate Extract of Andrographis paniculata on NF-κB Trans-Activation Activity and LPS-Induced Acute Inflammation in Mice

Table 1

Effects of AP EtOAc extract on pro-inflammatory cytokines production from LPS/IFN-γ stimulated Raw264.7 macrophage cells.

Raw264.7 macrophage cells
Treatment	TNF-α (pg ml^−1)	IL-6 (pg ml^−1)	MIP-2 (ng ml^−1)	NO (μM)
Medium only	n.d.	n.d.	n.d.	1.24 ± 0.25
LPS/IFN-γ (L/I)	717 ± 218	410 ± 95	1.48 ± 0.48	6.95 ± 1.21
L/I + Helenalin	n.d.	n.d.	n.d.	2.62 ± 0.62*
L/I + AP 2.5	667 ± 208	282 ± 98*	1.49 ± 0.72	3.29 ± 0.88*
L/I + AP 5	509 ± 185	275 ± 65*	1.39 ± 0.53	3.08 ± 0.80*
L/I + AP 10	388 ± 115*	100 ± 40*	1.16 ± 0.48*	3.03 ± 0.73*
L/I + AP 20	348 ± 62*	50 ± 10*	0.35 ± 0.05*	2.87 ± 0.73*

The data are expressed as the mean ± SD from three independent experiments. The significant difference compared with the control group was statistically by the Student's t-test (significant as compared with L/I alone, P < .05). Raw264.7 macrophages transient transfection with a NF-κB reporter plasmid were pretreated with 2.5–20 μg ml⁻¹ of AP EtOAc extract or helenalin (NF-κB inhibitor, 10 μM) for 1 h and then stimulated with L/I for 8 h. Supernatant were collected and analyses. n.d.: not detectable.