Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells

Figure 3

Effect of heat and enzymatic treatments on the anti-proliferative activity of ASH-WEX. The ASH-WEX was treated with heat (95°C), proteinase K (1 mg ml⁻¹), trypsin (1 mg ml⁻¹), DNase I (100 μg ml⁻¹) and RNase (100 μg ml⁻¹) for 30 minutes and its anti-proliferative and differentiation-inducing activity at concentrations ranging from 0.1 to –2.0% was evaluated in C6 glioma cells. Phase contrast images of cells (control (a)), and in the presence of heat and enzymatically treated ASH-WEX (c) showed no significant difference in the cell morphology. MTT assay of control and treated ASH-WEX showed similar anti-proliferative activity (b).

(a)

(b)

(c)