Original Article

Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells

Figure 6

Mortalin in response to ASH-WEX treatment. (a) Immunofluorescence detection of mortalin is shown in control, 0.5 and 1.0% ASH-WEX-treated C6 glioma cultures. Images were captured using Nikon E600 fluorescent microscope. (b) Relative intensity measurement of immunofluorescence. Significant change in mortalin expression in response to ASH-WEX-treated cultures compared with control is seen by (c) (P < .01) and (d) (P < .001). (c) Representative western blot hybridization signals for mortalin from control and test samples (0.5 and 1.0% ASH-WEX-treated cells). Single cell micrographs of mortalin immunofluorescence in control (i–iii) and 0.5% ASH-WEX-treated (iv–vi) cultures.