Carthamus tinctorius Enhances the Antitumor Activity of Dendritic Cell Vaccines via Polarization toward Th1 Cytokines and Increase of Cytotoxic T Lymphocytes
Figure 7
Ex vivo stimulation of specific T lymphocytes. The spleens of sham and tumor-bearing mice were taken and homogenized into single-cell suspensions. The splenocytes were re-stimulated with their corresponding treatments (DC vaccine or CT-treated DC vaccine) for 5 days. The splenocytes were then subjected to flow cytometric analysis, and specific T lymphocytes were gated by staining with FITC-anti-CD3 and PE-anti-CD8.
