Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from <i>Viscum articulatum</i> and Its Biological Effects on Peripheral Blood Mononuclear Cells

<table>Monitoring of soluble refolded ATB. (a) Circular dichroism (CD) spectra of rATB after refolding. Protein concentration: 0.05 mg/mL. (b) Hemagglutination activity of rATB. Wells: 1 PBS, negative control; 2–4 serially diluted refolded rATB. (c) rATB hemagglutination inhibition assay. Serially diluted Gal and GalNAc determined concentration needed for total inhibition of 30 <i>μ</i>g/mL rATB.</table>

Evidence-Based Complementary and Alternative Medicine

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Figure 4

Figure 4: Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from <i>Viscum articulatum</i> and Its Biological Effects on Peripheral Blood Mononuclear Cells 

Figure 4 | Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells 