Evidence-Based Complementary and Alternative Medicine / 2011 / Article / Fig 4

Research Article

CI431, an Aqueous Compound from Ciona intestinalis L., Induces Apoptosis through a Mitochondria-Mediated Pathway in Human Hepatocellular Carcinoma Cells

Figure 4

DAPI staining assay. The cells BEL-7402 were grown on poly-L-lysine-coated coverslips in 6-well plates and treated with CI431. After incubation for 24, the cells were stained with DAPI and then observed under the fluorescence microscopy. BEL-7401 cells in the control medium were stained homogeneously with DAPI (a), whereas treatment with CI431 led to marked chromatin condensation and nuclear fragmentation together with the appearance of small structures like apoptotic bodies. These observations indicated that the cells treated with CI431 entered apoptosis (b, c).

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