Evidence-Based Complementary and Alternative Medicine / 2011 / Article / Fig 5

Research Article

CI431, an Aqueous Compound from Ciona intestinalis L., Induces Apoptosis through a Mitochondria-Mediated Pathway in Human Hepatocellular Carcinoma Cells

Figure 5

Effect of CI431 on cell cycle distribution. BEL-7402 cells were seeded at 3 x 104/cm2 in 10-cm dishes and treated with CI431 at 20 (b), 40 (c) or 80 μg/mL, respectively or without treatment (a). After incubation for 24 h, cells were collected and the DNAs were stained by PI, while the RNAs were removed by digestion with RNase A. The DNA contents of the cells were determined with the FACSCalibur cytometer. The results showed that CI431 significantly induced a G2/S phase arrest with a decrease in G0/G1 phase population at 20, 40, and 80 μg/mL, while a dramatic increase of sub-G1 phase (hypodiploid cells) was observed at doses higher than 80 μg/mL.

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