Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells
Effect of LIEAF on the externalization of phosphatidylserine in Ca Ski cells. Ca Ski cells were treated without (control) or with 500 μg/mL of LIEAF for different time (24, 48, and 72 hours) and analyzed by annexin V/PI staining. (a) Flow cytometric fluorescence patterns of annexin V-PI staining. (b) Bar charts showed the percentage of distribution of viable, early apoptotic, late apoptotic and necrotic cells. (c) Results showed the percentage of annexin V positive cells. Data were means ± S.E. calculated from three individual experiments. The asterisk (*) represented significantly different from control (*).