Evidence-Based Complementary and Alternative Medicine / 2011 / Article / Fig 8

Research Article

Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells

Figure 8

Effect of LIEAF on the activation of caspase-3 in Ca Ski cells. (a) Ca Ski cells were treated without (control) or with varying concentrations of LIEAF for 24 hours. (b) In another experiment, Ca Ski cells were treated without (control) or with 500 μg/mL of LIEAF for 24, 48, and 72 hours. The DEVD-specific cleaving protease activity was then assayed in cell lysates using the caspase-3 assay kit as described in method. The activity of caspase-3 was detected by monitored the pNA liberated from Ac-DEVD-pNA, which was determined as fold increase compared to the control. The asterisk represented significantly different value from control (* ). Data were means ± S.E. calculated from three individual experiments.