A Chinese Herbal Decoction, Danggui Buxue Tang, Stimulates Proliferation, Differentiation and Gene Expression of Cultured Osteosarcoma Cells: Genomic Approach to Reveal Specific Gene Activation

Table 1

Genes related to bone development are up regulated by DBT.

Regulated gene^a	Symbol	Genbank no.	Fold of change^b
			DBT	RAS	RA
Lectin, galactoside-binding, soluble, 9 (Galectin-9)	LGALS9/Gal-9	AA434102	441.76	1.60	1.77
Small inducible cytokine subfamily A, member 8	SCYA8/CCL-8	AI268937	184.65	2.73	2.89
Small inducible cytokine A7	SCYA7/CCL-7	AA040170	175.02	0.33	6.96
Small inducible cytokine A2	SCYA2/CCL-2	AA425102	88.22	2.35	6.87
Collagen, type XVIII, α1	COL18α1	N81029	4.49	5.03	3.99
Matrix metalloproteinase 9	MMP9	T72581	3.87	5.78	7.15
Collagen, type X, α1	COL10α1	AI828306	3.44	4.09	13.95
Transforming growth factor, β2	TGFβ2	AA233738	2.60	3.40	1.77
Insulin-like growth factor 1	IGF1	AA456321	2.47	—	—
Bone morphogenetic protein 1	BMP1	R56774	2.29	2.88	6.66
Epidermal growth factor	EGF	AI480081	2.00	1.19	—
Integrin, α2	ITGα2	AA463257	1.87	0.75	0.85
Msh (Drosophila) homeo box homolog 2	MSX2	AA195636	1.86	1.14	0.82
Collagen, type VII, α1	COL7α1	AA598507	1.86	3.49	1.76
Bone morphogenetic protein 6	BMP6	AA424833	1.73	2.28	1.27
Msh (Drosophila) homeo box homolog 1	MSX1	AA464197	1.66	1.45	1.28
Fibroblast growth factor receptor 3	FGFR3	AA419620	1.65	0.97	0.82
Cartilage oligomeric matrix protein	COMP	N94385	1.64	1.30	4.82
Transforming growth factor, β1	TGFβ1	R36467	1.56	1.94	2.00
Bone morphogenetic protein 8	BMP8	AA779480	1.41	1.73	1.30
Bone morphogenetic protein 7	BMP7	W73473	1.39	1.75	1.64
Fibroblast growth factor receptor 1	FGFR1	R54846	1.36	1.17	1.25
Multiple inositol polyphosphate phosphatase 1	MINPP1	AA161161	0.29	0.47	0.57

^aGenes known to play role in bone development are selected for illustration; ^bChange of gene expressions as compared to the control (no drug treatment). “—” below detection.