Anti-Convulsant Activity of Boerhaavia diffusa: Plausible Role of Calcium Channel Antagonism

Table 2

Effect of different fractions of methanolic extract of B. diffusa on PTZ-induced convulsions in acute treatment studies.

Treatment/dose (mg kg^−1 )	Onset of convulsions (min) (mean ± SEM)	Number of animals showing convulsions	Severity score (mean ± SEM)	Mortality (%)
Vehicle control (75 mg kg^−1)	1.21±0.06	6/6	4.00±0.00	100
Diazepam (4 mg kg^−1)	NC	0/6	0	0
Diazepam (1 mg kg^−1)	2.13±0.07	6/6	3.66±0.23	66.6
Crude extract (1000 mg kg^−1)	3.97±0.11^a	4/6	3.66±0.21	66.6
Crude extract (1500 mg kg^−1)	4.28±0.06^a	3/6	3.33±0.33	50
Crude extract (2000 mg kg^−1)	11.33±0.46^𝑎	1/6	1.66±0.50^𝑎	16.6^𝑎
Chloroform fraction (20 mg kg^−1)	2.10±0.10	5/6	3.83±0.17	83.3
Phenolic compound fraction (1 mg kg^−1)	1.53±0.05	6/6	4.0±0.00	100
Liriodendrin-rich fraction (10 mg kg^−1)	2.49±0.19^a	4/6	3.33±0.33	66.6
Liriodendrin-rich fraction (20 mg kg^−1)	4.20±0.52^a	2/6	2.00±0.63^a	33.3^a
Liriodendrin-rich fraction (40 mg kg^−1)	3.07±0.05^a	0/6	1.83±0.48^a	0
Liriodendrin-rich fraction (20 mg kg^−1) + Diazepam (1 mg kg^−1)	NC	0/6	0	0

^a𝑃<.05 as compared with vehicle control group.