Cattle Bile Aggravates Diclofenac Sodium-Induced Small Intestinal Injury in Mice
Figure 3
Effects of DIF treatment and CB administration on COX-1 and COX-2 expression levels and PGE2 content in small intestine. Mice fed the control or CB diet were treated with DIF or vehicle, and the expression levels of COX-1 and COX-2 (a) and PGE2 content (b) in the small intestine were determined by Western blot analysis and enzyme immunoassay, respectively. Three and 6 mice in each group were used for the determination of COX expression levels and PGE2 content in the small intestine, respectively. Fold difference in densitometric values of COX-1 bands compared with that of untreated group were 2.9, 3.0, and 5.7 in the CB-, DIF-, and CB/DIF-treated groups, respectively. PGE2 contents in the small intestine is expressed as mean + SEM. The probability values estimated in two-way analysis of variance were 0.0634, 0.0004, and 0.0634 for the effects of DIF treatment, CB administration, and their interaction, respectively.
