Administration of a Decoction of Sucrose- and Polysaccharide-Rich Radix Astragali (Huang Qi) Ameliorated Insulin Resistance and Fatty Liver but Affected Beta-Cell Function in Type 2 Diabetic Rats
Figure 3
Liver histopathology and hepatic protein level analysis after the water extract of Radix Astragali (AM-W) administration. (a) At the end of AM-W administration, representative liver histopathology was examined and illustrated. Representative pictures from photography, H&E staining, oil-red O staining, periodic acid-Schiff staining, and Masson’s trichrome of control (ND), type 2 diabetes (T2D), and T2D + AM-W groups are shown. In addition, hepatic proteins from each group were extracted to measure the expressions of (b) AMP kinase (AMPK) phosphorylated at Thr172 (pAMPK), (c) phosphoenolpyruvate carboxykinase (PEPCK), (d) acetyl CoA carboxylase (ACC), and (e) fatty acid synthase (FAS). Each blot was quantified, converted to the relative ratio of actin, and presented as the mean ± SEM ( in each group). Differences were determined by ANOVA followed by the Tukey-Kramer test. * was considered significant when compared to ND rats. ∆ was considered significant when T2D and T2D + AM-W rats were compared.
