Neuron Regeneration and Proliferation Effects of Danshen and Tanshinone IIA

Figure 3

Exaimination of cell cycle signaling (a), activated FGF signaling (b, c), and Schwann cell migration of danshen and tanshinone IIA on RSC. RSC cells were incubated with different doses of danshen and tanshinone IIA as indicated for 16 hrs. Cells were then lysed. Protein was extracted and analyzed using western blotting. α-tubulin was used as a loading control. (c) Matrix metalloprotease-9 and -2 enzyme activity was examined using zymography. (d) The Schwann cell migration was examined using the Boyden chamber system. The number of cells that had migrated were quantitated using the mean value of 10 microscopic fields in every condition. *, **, and *** denote significant differences between experimental and control values, with 𝑃<.05, 𝑃<.01, and 𝑃<.001.

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