Figure 2: Analysis of the effect of Florin extracts on apoptosis in TCCSUP cells. (A) Cells were treated with 0, 6, 12, or 25 μg/mL of Florin extracts. After 18 h, the percentage of early apoptotic cells was assessed by Annexin-V/PI binding assay. (B) After 24 h, the adherent cells or suspension cells were collected and subjected to DAPI staining. (C) The ratio of DAPI-stained cells was calculated and the data are presented as mean ± S.D. * , ** . (D) After the cells were treated with 0, 6, 12, and 25 μg/mL of Florin extracts for 48 h, the PARP protein was resolved on 10% SDS-PAGE and then Western blot was performed. GAPDH was used as a control.