Table 2: Flow cytometry analysis in TCCSUP cells treated with Florin extracts.

Time (h) Percentage (mean ± S.D.) of TCCSUP bladder cancer cells at each cell cycle phase after treatment with Florin extract (25 μg/mL) at different time points

subG1 (%)G1 (%)S (%)G2M (%)

00.5 ± 0.155.9 ± 3.918.2 ± 2.825.4 ± 1.9
60.6 ± 0.424.3 ± 1.615.1 ± 3.3 59.6 ± 2.1**
120.7 ± 0.12.9 ± 0.514.1 ± 0.5 82.3 ± 0.1***
241.7 ± 0.71.7 ± 0.62.0 ± 0.2 94.6 ± 0.1***
364.7 ± 2.03.0 ± 1.74.2 ± 1.6 88.1 ± 1.5***
4815.4 ± 2.33.4 ± 0.55.6 ± 0.4 75.6 ± 3.3***

aTCCSUP cell growth was arrested at the G2/M phase after Florin-extracts treatment in a time-dependent manner, and these cells were significantly different from those at baseline (0).
bThere was a significant difference between these cells and those in the control group (** , *** ).