The effect of five distinct fractions (1/5, 1/3, 1/2, 2/3, 4/5) on Hep-G2 viability (a)–(e). (f) corresponds to the LC50s values of GE, LE and five fractions. Bars with different letters are significantly different from each other (). Results are expressed as mean ± SD of five separate experiments with five replicates.