DNA cell-cycle histograms of Hep-G2 cells. Cells were treated with GE, LE and five fractions (1/5, 1/3, 1/2, 2/3, 4/5) for 24, 48 and 72 h, respectively, at the respective LC50s. Untreated cells acted as controls. Cells were fixed in 70% ethanol and stained with PI as described in the Methods section. DNA histograms shown are representatives of the assay repeated in three independent experiments with similar results.
